Supplemental Data Yeast H2A.Z, FACT complex and RSC regulate transcription of tRNA gene through differential dynamics of flanking nucleosomes
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چکیده
High resolution MNase (micrococcal nuclease) footprinting (Figure S1) shows TFIIIC binding to chromatin but no nucleosomal footprint is seen on the transcribed region of the gene, suggesting unlike SNR6 (24), gene is not covered by a positioned nucleosome. A chromatin-specific MNase hypersensitivity downstream of box B probably denotes the boundary of a positioned nucleosome downstream, covering the gene terminator.
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Yeast H2A.Z, FACT complex and RSC regulate transcription of tRNA gene through differential dynamics of flanking nucleosomes
FACT complex is involved in elongation and ensures fidelity in the initiation step of transcription by RNA polymerase (pol) II. Histone variant H2A.Z is found in nucleosomes at the 5'-end of many genes. We report here H2A.Z-chaperone activity of the yeast FACT complex on the short, nucleosome-free, non-coding, pol III-transcribed yeast tRNA genes. On a prototype gene, yeast SUP4, chromatin remo...
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